Grower Implemented Quantitative LAMP for Initiating and Adjusting Fungicide Program

The mission of this research is to increase the economic sustainability of grape production by providing decision support tools to aid in management of grape powdery mildew. In this project we propose to test the utility of a quantitative Loop mediated isothermal AMPlification assay (qLAMP) and handheld device for detection and quantification of airborne inoculum; thereby extending our  esearch on the use of inoculum detection as a decision support tool for managing grape powdery mildew. The specific objectives are:

  1. Test implementation of a grower preformed quantitative LAMP assay.
  2. Examine the effectiveness of adjusting fungicide interval based inoculum density.
  3. Assessment of quantitative LAMP (qLAMP) for estimating amount of fruit infection

Results: Due to reduced funding, Objective 1 had to be reduced and Objective 3 eliminated in the second year of this 3 year project. The extensive cooperation of several participating growers who shuttled samples from the upper Willamette Valley to Corvallis, allowed for us to complete Objective 2 using lab processed samples only.