Research Results
Click a title below to view a research summary or narrow your search by entering a specific search term or topic. You can find research summaries keyword, funding year, primary investigator or category using the search boxes at right. Project summaries are available for every year since 1991.
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Building Community Resilience, Coordination, and Collaboration for Individual and Regional Red Blotch Disease Management. AVF Project #2024-2746.
The goal of this project was to increase the adoption of grapevine red blotch disease (GRBD) management practices at both an individual and regional scale. To accomplish the goal, this outreach project supported and empowered viticulture professionals to successfully advocate for the adoption of GRBD management practices within their organizations and outside their organizations when engaged in collective action with neighboring vineyards.
Extension and Outreach through UC Davis Viticulture and Enology On-the-Road Events. AVF Project #2025-1685.
By the end of the 2024-2025 funding cycle, we completed three of the four On the Road (OTR) educational events originally proposed in the 2024 AVF Extension and Outreach proposal (Temecula, Sonoma, and Napa Counties). We used NCE 2023-1685 to fund the program in Temecula in November 2024.
Improving Biological Control of Vine Mealybug. AVF Project #2025-2982.
The principal goal of this project is to optimize control of vine mealybug (VMB) by identifying and then manipulating factors that impact natural enemies of VMB. Severe VMB infestations can reduce grape vine vigor and fruit marketability.
Unveiling the Spatial-Temporal Impact of Red Blotch on Grape Composition and Grapevine Physiology and Exploring Mitigation Strategies Via Cultural Practices. AVF Project # 2025-2964.
Grapevine Red Blotch Virus (GRBV) is a disease that can slow grape ripening and reduce fruit quality, creating economic challenges for vineyard owners and winemakers. During the first year of this project, our team focused on understanding how this virus affects grape sugar levels, acidity, and vine health in real commercial vineyards, and on testing practical ways growers might reduce its impact.
The Role of Rootstocks, Single and Mixed Infections of Grapevine Leafroll Associated Virus-3, and Grapevine Virus-A in Sudden Vine Collapse. AVF Project #2025-2561.
This project was planned to determine if co-infections of grapevine leafroll associated virus-3 (GLRaV-3) and grapevine virus A (GVA) lead to sudden vine collapse (SVC) on Freedom rootstock and to identify rootstocks that might be more resistant.
Highly Selective Enzymatic Degradation of Undesirable Phenols in Brettanomyces and Smoke Taint Contaminated Wines. AVF Project #2025-2795.
We have completed the third year of a multi-year project focused on developing enzymes that selectively degrade 4-ethylguaiacol (4EG) and 4-ethylphenol (4EP), the primary sensory-active compounds in Brettanomyces-contaminated wine and a subset of the broader taint phenols present in smoke-tainted wine (Licker 1998; Mirabelli-Montan 2021). A second phase of this project, beyond the scope of the current proposal period, will extend the same engineering framework to additional smoke taint marker compounds, including guaiacol, 4-methylguaiacol, cresols, syringol, and 4-methylsyringol.
Chemistry, Mouthfeel, and Consumer: Understanding Red Wine Astringency via Sensory and Analytical Approaches (Year 1). AVF Project #2025-2954.
Wine astringency is a major driver of red wine style and consumer experience, yet it remains hard to predict when wines appear similar by routine tests. Phenolic compounds are chemically diverse, and their effects depend on molecular structure and interactions with the wine matrix and saliva, so wines with similar pH, ethanol, and conventional total phenolic measurements can still feel meaningfully different.
Managing Tannins in the Winery. AVF Project #2025-2978.
To further investigate the structural changes to grape tannins and the impact on grape tannin concentration by glutathione additions, an experimental design was carried out with red wine made to two separate maceration lengths and glutathione added at multiple time points throughout the winemaking process.
Leveraging Berry Physiology to Mitigate Late-Season Dehydration. AVF Project #2025-2739.
In Years 1 and 2, we tested whether short (2-week) pulses of increased irrigation before or at the expected onset of cell death would reduce water stress-induced oxidative damage and delay or reduce the rate of cell death, reducing shrivel at harvest. We found that the irrigation pulse at onset significantly reduced the rate of cell death and shrivel at harvest, providing irrigation guidelines to reduce late-season dehydration.
Integrating a Novel Method to Estimate Grapevine Water Use and Stress into Multi-Tiered Precision Irrigation Management. AVF Project #2025-2796.
We continued our efforts on this project to test and refine the use of IRT wavelet method as a method to track vine water use and stress across a variety of field sites and vineyard settings. In year 2, we repeated a second year of study in experimental vineyard located in Davis with variable rate irrigation system, where we conducted a drought experiment with heavily instrumented and measured vines.
Advancing Elite Grape Rootstocks with Reduced Nematode Susceptibility. AVF Project #2025-2669.
Vineyards suffer from infestations with plant-parasitic nematodes. Owed to the extended years of cropping vineyards, the soil-dwelling parasites have ample opportunities to build up and cause damage. Thus plant-parasitic nematodes can become major yield constraints. A single location is often infested by multiple species including Meloidogyne spp. (root-knot nematodes), Mescocriconema xenoplax (ring nematode), Xiphinema americanum or X. index (dagger nematode), and Tylenchulus semipenetrans (citrus nematode).
Integrating Genomics and Phenomics Tools for Improved Grapevine Rootstock Breeding. AVF project #2025-2953.
California grape production increasingly faces challenges from drought, soil salinity, micronutrient toxicity, viruses, and nursery production constraints. Rootstocks are the primary tool growers use to mitigate these stresses, yet the genetic diversity of commercially available rootstocks remains limited. This project aims to accelerate the development of improved grapevine rootstocks by integrating long-term field trials with modern genomic and high-throughput phenotyping tools.
Can Water-Saving Traits Reduce Grapevine Irrigation Demand Without Compromising Yield and Quality? AVF project #2024-2811.
The goal of our project is to use genetic engineering to generate plant material with water-saving stomatal traits, while leaving the rest of the genome intact, to experimentally test the effects of these traits on yield and ripening in economically important grape cultivars
Genome Editing of Grape Cytidine Diphosphate Diacylglycerol Synthases to Confer Broad-Spectrum Disease Resistance. AVF project #2024-2873.
Full-length cDNAs of two grapevine cytidine diphosphate diacylglycerol (CDP‐DAG) synthase (CDS) genes VvRBL1-1 (Vitvi10g00174) and VvRBL1-2 (Vitvi19g00570) were cloned from Thompson Seedless leaves. Both VvRBL1-1 and VvRBL1-2 were proposed as genome editing targets for enhancing broad-spectrum disease resistance in grapevine.
Assessment of the Variability in Soil Health Indicators and Incorporating Healthy Soil Management Practices in Napa Valley, Paso Robles, and Lodi regions. AVF Project #2022-2722.
Adoption of sustainable soil management practices is becoming common in winegrape production in response to an increased awareness of the value of soil health to maintain environmental quality. Soil health is characterized by the functions it provides to the vineyard (i.e water retention, nutrient supply, carbon sequestration etc.) and is often impacted by vineyard soil management practices (i.e., compost application, no till, cover crops). However, the magnitude of the effect of these practices on soil organic matter and other soil health indicators is difficult to predict. A major impediment for the diagnosis and establishment of target values of soil health in vineyards is the large diversity of soils and physiographic conditions across and within growing regions.
Spray-induced Silencing of Grape Powdery Mildew Genes to Reduce Powdery Mildew Growth. AVF Project #2023-2366.
Powdery mildew is the dominant disease of CA grapevine. It infects all grape varietals with vines treated ~9-11 times over the growing season. Coupled with increased restrictions on synthetic chemical fungicide use and the demand for safer, more sustainable treatment options is the reduced efficacy of synthetic chemical fungicides due to the development of resistance by the powdery mildew fungi.
Integrating a Novel Method to Estimate Grapevine Water Use & Stress into Multi-Tiered Precision Irrigation Management. AVF Project #2023-2796.
Our efforts during the 2024 field season focused on Objective #1 using a combination of existing datasets, continuation of data collection in various GRAPEX sites, and installation of new sensors in an experimental vineyard in Davis. In 2025, we will also utilize large, mature vines growing in harvest collecting bins that are sitting on load cells. This system will allow us to track water use continuously and compare ET measured with the IRT wavelet sensor.
From the Lab to the Field: Improving Biocontrol Strategies Against Trunk Diseases in Vineyards. AVF Project #2024-2689.
Summary of Progress:
Multiple strategies involving biocontrol…
Evaluating Alternative Nursery Practices to Reduce Graft Union Failure. AVF project #2024-2692.
This is the second year of the project focused on evaluating alternative nursery practices to reduce graft union failure in grapevine propagation. This study examined how nursery practices influence graft union success and disease management in grapevine propagation.
Variable Rate Irrigation Scheduling Based on High-Resolution Short-Wave Infrared Sensing and Internet-of-Things. AVF Project #2023-2718.
Amidst escalating drought conditions in the San Joaquin Valley, grapevine growers face an urgent need for effective water management strategies. This research contributes valuable insights into sustainable viticulture practices, particularly for optimizing water use in vineyards while addressing the physiological responses of grapevines to varying irrigation intensities and timings.
On-the-Go Mapping of Grape Composition in the Field Through Hyperspectral Machine Vision. AVF Project #2022-2607.
This project aimed to develop a non-invasive, real-time method for assessing grape composition in vineyards using hyperspectral imaging technology. This innovative approach helps viticulturists monitor grape quality and make informed decisions about vineyard management and harvesting.
Extension and Outreach through UC Davis Viticulture and Enology On-the-Road. AVF Project #2024-1685.
By the end of the 2023-2024 funding cycle, we completed three of the four On the Road (OTR) educational events originally proposed in the 2023 AVF Extension and Outreach proposal (Alameda, San Luis Obispo, and Kern Counties) plus the program originally scheduled in San Diego, was postponed (with NCE 2023-1685), to November 2024. We used the NCE to fund the San Diego/Temecula program which took place on November 20, 2024. We have now completed all the events proposed in the 2023-24 grant (Proposal 2023-1685).
Can Water-Saving Traits Reduce Grapevine Irrigation Demand Without Compromising Yield and Quality? AVF Project #2024-2811.
Climate change is expected to increase irrigation demand at the same time as reducing irrigation supply, and developing plant material with lower irrigation needs is crucial to adapt the grape industry to future conditions. Previous work suggests that altering stomatal traits to reduce maximum gas exchange rates or reduce gas exchange more strongly in response to water stress would increase grapevine water-use efficiency without compromising the carbon supply for growth and ripening.
Leveraging Berry Physiology To Mitigate Late-Season Dehydration. AVF Project #2024-2739.
Hot, dry conditions can exacerbate late-season berry dehydration, reducing yield and altering berry sensory properties. Late-season dehydration occurs when the berries undergo programmed cell death, and the water released from the ruptured cells is lost to transpiration or backflow to the canopy. Climate change is expected to make many wine regions warmer and drier, exacerbating these issues.
The role of rootstocks and single and mixed infections of grapevine leafroll associated virus-3 and grapevine virus A in sudden vine collapse. AVF Project #2024-2561
This project was planned to determine if co-infections of grapevine leafroll associated virus-3 (GLRaV-3) and grapevine virus A (GVA) lead to sudden vine collapse (SVC) on Freedom rootstock and to identify rootstocks that might be more resistant.
Highly Selective Enzymatic Degradation of Undesirable Phenols in Brettanomyces and Smoke Taint Contaminated Wines. AVF Project #2024-2795.
We've completed the second year of a multi-year project with significant progress in developing enzymes capable of selectively degrading 4-ethylguaiacol (4EG) and 4-ethylphenol (4EP), the primary sensory-active compounds in Brettanomyces-contaminated wine and responsible for a portion of the ‘taint’ phenols found in smoke-tainted wine (Licker 1998, Mirabelli-Montan 2021).
Furthering Elite Grape Rootstocks with Reduced Nematode Susceptibility. AVF Project #2024-2669.
Vineyards are continuously at risk for infections by soil-borne pests and pathogens which have ample time and opportunity to establish, build-up in population densities, and cause damage. Depending on the severity of infection, plant-parasitic nematodes can become major yield constraints.
Rapid Evaluation of Smoke exposure in Grapes and Wine by Raman Spectroscopy – A Concept Proposal
Raman Spectroscopy of Smoke Compounds
We have evaluated ten different smoke compounds in synthetic wine using a 785 nm Wavelength Raman setup using defined parameters to gain spectra. Individual compounds and combinations were tested to provide us with initial results showing the various Raman fingerprints of the smoke compounds and help determine any potential interferences between them.
Wine Compound Interactions That Cause Perceived Smoke Taint in Red Wines.
As detailed in the previous years report more than 120 wines were collected that have various levels of smoke taint. The majority of wines (~100) were Cabernet Sauvignon and Pinot noir, but a few other varietals were also included such as Petit Verdot, Merlot and Syrah. Sensory analysis for these wines were completed in August 2023.
Achieving tropical fruit aroma in white wines: from winemaking to consumer acceptance.
The overall goal of this project is to produce Chardonnay wines with increased tropical fruit aroma perception. In a previous study developed by Dr. Elizabeth Tomasino’s research group, we found that wines with higher concentrations of fermentation esters and volatile thiols imparted more intense tropical fruit aroma nuances.
Effector-assisted breeding against powdery mildew in grapes.
In last three decades, a great deal of effort and resources have been placed by the grape breeding community on the identification of resistance (R) loci against the grape powdery mildew (GPM) pathogen Erysiphe necator, and their introgression in grapevine varieties of interest.
Highly Selective Enzymatic Degradation of Undesirable Phenols in Brettanomyces and Smoke Taint Contaminated Wines.
This is the first-year progress report on a multi-year project divided into two-phases with the first (current) phase focusing on discovery and design of enzymes with exclusive degradation of 4-ethylguaiacol (4EG) and 4-ethylphenol (4EP), the main sensory-active components in wine contaminated with Brettanomyces and responsible for a portion of the ‘taint’ phenols found in smoke-tainted wine (Licker 1998, Mirabelli-Montan 2021).
Rapid evaluation of smoke exposure in grapes and wine by Raman spectroscopy and machine learning.
We have evaluated 10 different smoke compounds in synthetic wine using a 785 nm Wavelength Raman setup using defined parameters to gain spectra. Individual compounds, as well as different combinations, were tested to provide us with initial results showing the various Raman fingerprints of the smoke compounds and help determine any potential interferences between them.
Impact of malolactic fermentation timing and the use of non-Saccharomyces yeast during cold soaking on Pinot noir wine color and sensory properties. (2024-2704
The malolactic fermentation (MLF) is a key process in the production of red wines and some white wines. While it is commonly conducted after the completion of the alcoholic fermentation (sequential), it can also be induced at the same time where Oenococcus oeni is inoculated shortly after the beginning of alcoholic fermentation (AF).
Evaluating alternative nursery practices to reduce graft union failure.
Graft failure can be caused by factors such as the poor formation of the graft union (due to problems with anatomical mismatching, poor grafting technique, adverse weather conditions, and poor hygiene), mechanical damage, and graft incompatibility.
From the Lab to the Field: Using Endophytic Bacteria as Biocontrol Agents Against Trunk Diseases in Vineyards.
Bacterial isolates selected from previous AVF grants (2019-2332 and 2021-2573) alongside other beneficial fungal isolates were investigated as potential biocontrol agents against grapevine trunk diseases pathogens. The biocontrol agents were applied as pruning wound protectants (i), as dipping treatments of propagation material prior grafting in nurseries (ii), and as soil drench applications (iii).

